Journal: Cell & Bioscience

Article Title: Characterization of a migrative subpopulation of adult human nasoseptal chondrocytes with progenitor cell features and their potential for in vivo cartilage regeneration strategies

doi: 10.1186/s13578-016-0078-6

Figure Lengend Snippet: The differentiation potential of mnCPC was compared to hCh and MSC and induced by growth factor supplemented differentiation media over a period of 21 (adipogenic and osteogenic differentiation) and 28 days (chondrogenic differentiation). Only MSC, cultured in adipogenic differentiation medium, exhibited fatty vacuoles positive with Oil red O staining ( a ). Strong calcium deposition—stained with von Kossa stain—was shown for MSC and mnCPC, only small deposits were found in hCh ( b ). In the pellet culture for chondrogenic differentiation, collagen type II was detected in all samples ( c ). All control groups, cultured in basal medium, were negative ( d ). The respective gene expression analysis ( e – g ) supported the findings visualised by histological and immunohistochemical staining. Graphs show individual values with median, n = 4–8 donors for each cell type, *p ≤ 0.05. Representative images were chosen

Article Snippet: The initial cell density was 3.15 × 10 4 cells cm −2 in MSC adipogenic differentiation medium (PromoCell, Heidelberg, Germany).

Techniques: Cell Culture, Staining, Expressing, Immunohistochemical staining